Agenda for next week
Monday
rodZ-gfp construct
Digest yapC and rodz PCR products with SpeI
Digest gfp with XbaI
Inactivate at 70degreesC for 30minutes.
Ligate overnight.
Plac-gfp control
Digest Plac miniprep with SpeI and PstI
Digest gfp with XbaI and PstI
Ligate overnight
Tuesday
Transformation with Plac-gfp ligation
Fusion PCR of yap-gfp and rodz-gfp
Wednesday
Digest Plac miniprep with SpeI and PstI
Digest rodz-gfp with XbaI and PstI
Ligate overnight.
Thursday
Transform with Plac-rodz-gfp overnight.
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