Synthetic Morphology for Tissue Engineering

Gel analysis of ligation reactions
The ligation reactions for 1) yapC-gfp 2) rodz-gfp and 3) plac-gfp in pSB1A2 plasmid, did not show any bands. The DNA ladder was visible and I adjusted the settings to increase exposure but the lanes for the ligations were as clean as a sheet. I suspect that this is due to low amounts of DNA as unligated fragments were also not visible.

I think we should just go ahead with the PCR reactions using yapC forward + gfp reverse, rodZ forward + gfp reverse. If we get PCR products, it will indicate that the ligations worked. Also, positive results will allow us to be ‘confident’ that plac-gfp ligation also worked before we proceed with transformation.