Synthetic Morphology for Tissue Engineering

Proof-of-concept experiment in E. coli

Today: RodZ-GFP PCR products

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FINALLY, we got the promega 5 minute ligation kit to work. The trick (for me) was to clean up the digestion products before ligation. I suppose the ligase is really sensitive to the 5x ligase buffer.

Gel purification awaits.

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Written by lifein553

February 4, 2010 at 3:41 pm

Posted in Today

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