Synthetic Morphology for Tissue Engineering

Proof-of-concept experiment in E. coli

Reading/ Thoughts

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Is there a way to quantitatively measure rodZ expression. Direct method, not based on GFP.

Alyahya et al. used 10h growth in arabinose, 2h IPTG

Microscopy
Light microscopy Cells were spotted on a 1% agarose-padded slide containing appropriate antibiotics or inducers.
Fluorescence microscopy Grown in LB or M9 supplemented with 0.2% glucose, 1ug/ml thiamine and 0.05% casamino acids

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Written by lifein553

February 18, 2010 at 11:46 am

Posted in Reading

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